BcII RFLP for the human vimentin gene

A TaqI RFLP for the human arylsulfatase A gene.

A frequent Hindlll RFLP of the human fibrorrertin gene ( FNl )

SOURCE/DESCRIPTION: The probe used is a Hindlll/BamHI fragment of 1.4kb from the fibronectin recombinant, pFH6 (Kornblihtt, A.R. et al.). POLYMORPHISM: Hindlll (A/AGCTT) (New England, Bio-Labs, FRG) detects invariant bands at 4.75 and 1.7kb in addition to a simple two-allele polymorphism defined by the presence of a band of 7.1kb (F2) alternatively with two bands of 3.7 and 3.4kb (Fl) (Figure 1...

MspI RFLP in the human fumarylacetoacetate hydrolase (FAH) gene.

StuI RFLP in the human ABL locus.

Source/Description: A 3.6 kb EcoRI fragment (lambda ABL3) containing portions of exons 7 and 8 and the intervening intron of the ABL oncogene was isolated from a chromosome 9-specific library (ATCC # LA09NS01) using a 1.7 kb Abelson murine leukemia virus probe (v-abl, ONCOR, Inc.). A single copy 1.8 kb BglII fragment (ABL3) was derived from the original lambda clone and detects the StuI RFLP. P...

Improved visualization of the Bst EII RFLP of the human LDL receptor gene by co-digestion.

Bst EII identifies, a bi-allelic polymorphism in the human LDL receptor gene (l). The 33kb allelc cannot readily be distinguished from the 24kb fragment in the heterozygote using a 0.6 agarose gel. Lower percentage gels often cause handling problems. We suggest co-digestion of DNA with non-polymorphic enzymes such as Eco RI (or Bam HI). Figure 1 shows an autoradiograph of a Southern blot where ...